Use of West-Western Blotting for Detection of Adenovirus Receptors

L. A. Tarassishin^1,2 and W. C. Russell³

¹Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, Kiev, 252143 Ukraine; fax: (044) 266-23-79; E-mail: leo@virol.imv.kiev.ua or smirnov@imv.kiev.ua

²To whom correspondence should be addressed.

³Division of Cell and Molecular Biology, School of Biological and Medical Sciences, University of St. Andrews, St. Andrews, Scotland, UK.

Submitted March 29, 1996; revision submitted October 10, 1996.

A modification of an immunoblotting procedure was developed to detect virus receptors: a nitrocellulose membrane containing electrotransferred cellular proteins separated by polyacrylamide gel electrophoresis in the presence of SDS is treated with the whole virus (viral proteins) and an antiserum against individual viral proteins, followed by the detection of the receptors after their incubation with peroxidase labeled anti-species antibodies by enhanced chemiluminescence. The method detects adenovirus receptors for the capsid proteins including 20- and 80-kD receptors for the fiber, 20- and 40-kD receptors for the penton, and 80- and 200-210-kD receptors for protein IIIa.

KEY WORDS: virus receptor, adenovirus, west-western blotting.

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