Overexpression of the Gene of Ribosomal Protein L30 from Thermus thermophilus and Crystallization of the Recombinant Protein

A. R. Khairullina,^1,2 D. V. Shcherbakov,¹ S. V. Tishchenko,¹ S. V. Nikonov,¹ and M. B. Garber¹

¹Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia; fax: (7-095) 924-04-93; E-mail: protres@sovam.com

²To whom correspondence should be addressed.

Submitted October 24, 1996; revision submitted November 21, 1996.

Ribosomal protein L30 from Thermus thermophilus was overexpressed in E. coli cells. The recombinant protein was isolated and crystallized. The crystals belong to the spatial group P3₁12, and their crystallographic parameters are not different from those of crystals obtained earlier from the ribosomal protein isolated from T. thermophilus.

KEY WORDS: ribosomal proteins, overexpression, crystallization, Thermus thermophilus.

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