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Site-Specific DNA-Methylase M·BspST5I Methylates Only One Strand of the Recognized Site

N. N. Matvienko,1 L. A. Zheleznaya,2 N. V. Zelinskaya,3 and N. I. Matvienko3,4

1Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia.

2Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia; fax: (7-095) 135-62-19.

3Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia; fax: (7-095) 924-04-93; E-mail: protres@sovam.com

4To whom correspondence should be addressed.

Submitted October 24, 1996; revision submitted December 16, 1996.

We recently isolated a site-specific adenine DNA-methylase, M·BspST5I, which methylates only one strand of the recognized site GC***ATC. The methylated base is indicated by an asterisk.

KEY WORDS: site-specific DNA-methylase, restriction-modification, S-adenosyl methionine.