Preparation and Characterization of Monoclonal Antibodies to Foot-and-Mouth Disease Virus Type Asia-1

S. P. Amineva,^1,2 N. L. Davydova,¹ L. U. Mamatkulova,¹ L. A. Globenko,¹ and V. A. Mishchenko¹

¹Institute of Animal Health, Russian Ministry of Agriculture and Food, Yur'evets, Vladimir Region, 600900 Russia; fax: (09222) 60-614 or (09222) 37-261.

²To whom correspondence should be addressed.

Submitted May 25, 1995; revision submitted February 13, 1997.

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Eight clones of hybrid cells secreting monoclonal antibodies to foot-and-mouth disease virus type Asia-1 were prepared; three of them neutralized viral infection. The specificity of THE monoclonal antibodies was analyzed by various immunoenzyme assays using 146S viral particles, trypsin-treated 146S particles, 12S particles, and certain viral polypeptides. The epitopes unique for virus type Asia-1 and conservative among several types were detected on the surface of viral particles. Epitopes on the surface of viral particle were mapped.

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KEY WORDS: foot-and-mouth disease virus, monoclonal antibody, antigenic determinant.

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