Bioluminescence of Free and Poly(vinyl alcohol) Cryogel-Entrapped Recombinant Escherichia coli Cells Expressing Firefly Luciferase

N. A. Romanova, L. Yu. Brovko, T. A. Makhlis, E. M. Sinodova, and N. N. Ugarova^*

School of Chemistry, Lomonosov Moscow State University, Moscow, 119899 Russia; fax: (095) 939-5417; E-mail: UNN@enzyme.chem.msu.su

^* To whom correspondence should be addressed.

Received September 26, 1997

---

Bioluminescence of free and poly(vinyl alcohol) cryogel-entrapped recombinant E. coli cells expressing firefly luciferase was investigated. It was shown that bioluminescence intensity and time-course of the bioluminescent signal changed upon immobilization and depended on intracellular ATP concentration and permeability of the cell membrane.

---

KEY WORDS: bioluminescence in vivo, firefly luciferase, recombinant E. coli cells, immobilization, poly(vinyl alcohol) cryogel, polymyxin, pH-shift

---