Structure of Sulfated O-Specific Polysaccharide of the Marine Bacterium Pseudoalteromonas marinoglutinosa KMM 232

N. A. Komandrova^*, S. V. Tomshich, V. V. Isakov, and L. A. Romanenko

Pacific Institute of Bioorganic Chemistry, Far East Division of the Russian Academy of Sciences, pr. 100-letiya Vladivostoka 159, Vladivostok, 690022 Russia; fax: 7 (4232) 31-4050; E-mail: elnaz@piboc.marine.su

^* To whom correspondence should be addressed.

Received November 10, 1997; Revision received January 18, 1998

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A sulfated O-specific polysaccharide containing D-mannose, L-rhamnose, and the sulfate group was obtained by mild acid hydrolysis of lipopolysaccharide (S-form) of the marine bacterium Pseudoalteromonas marinoglutinosa KMM 232. Based on analysis of methylation and ¹³C-NMR spectroscopy of native and desulfated polysaccharides, the following structure of disaccharide repeat unit in the O-specific polysaccharide has been established:

This is the first report of a sulfated O-specific polysaccharide isolated from gram-negative bacteria.

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KEY WORDS: Pseudoalteromonas marinoglutinosa, marine bacteria, lipopolysaccharide, sulfated polysaccharide, structure, NMR spectroscopy

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