Purification and Characterization of P33 Protein of Candida utilis Homologous to Bgl2p of Saccharomyces cerevisiae; Comparative Analysis of the Role of These Proteins in Molecular Organization of the Yeast Cell Walls

T. S. Kalebina^1*, D. K. Laurinavichyute¹, A. B. Shevelev², G. V. Fominov², E. I. Levitin², O. V. Alekseeva¹, Sipin Chzhan¹, N. V. Pan'kova³, V. N. Ksenzenko³, V. M. Stepanov², and I. S. Kulaev¹

¹School of Biology, Lomonosov Moscow State University, Moscow, 119899 Russia; fax: (095) 939-4658

²State Research Institute of Genetics and Selection of Industrial Microorganisms, 1-yi Dorozhnyi Proezd, Moscow, 113545 Russia; fax: (095) 315-0501

³Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia; fax: (095) 923-3602

^* To whom correspondence should be addressed.

Received February 23, 1998

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P33 protein was isolated from the cell walls of Candida utilis. Homology between P33 and Bgl2p proteins from the cell walls of Saccharomyces cerevisiae was shown. The important role of these proteins in molecular organization of yeast cell walls was demonstrated using trypsin proteolysis and the "gene disruption" method.

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KEY WORDS: cell wall, yeast, cell wall proteins, proteinases

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