2Laboratoire de Chimie Biologique et UMR 8576 CNRS, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq, France; fax: (33-320) 43-6555
* To whom correspondence should be addressed.
Received September 29, 1998; Revision received February 4, 1999
Amphibian oviduct and liver were shown to contain enzymes that catalyze the formation of GDP-beta-L-fucose from GDP-alpha-D-mannose or L-fucose. The conversion of L-fucose into beta-L-fucopyranosyl phosphate was achieved on a preparative scale using high-activity fucokinase in toad liver extracts. For chemo--enzymic preparation of GDP-beta-L-fucose, a convenient modification for pyrophosphate synthesis through phosphomorpholidate which does not require anhydrous conditions is suggested.
KEY WORDS: fucosyl phosphate, guanosine diphosphate fucose, chemo--enzymic synthesis, amphibian tissues