Influence of Ethanol and Phosphatidylethanol on the Activity of Pancreatic Phospholipase A₂

M. A. Kisel, S. V. Kuchuro, and N. M. Litvinko^*

Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, ul. Akademika Kuprevicha 5/2, Minsk, 220141 Belarus; E-mail: kisel@ns.iboch.ac.by

^* To whom correspondence should be addressed.

Received December 22, 1999; Revision received July 5, 2000

---

Hydrolysis of dioleoylphosphatidylethanol (DOPEt) and dioleoylphosphatidylcholine (DOPC) catalyzed by phospholipase A₂ (PLA₂) from porcine pancreas has been studied in single-component and binary liposomes in the absence and in the presence of ethanol. DOPEt (an anionic phospholipid) was found to increase the rate of hydrolysis of zwitterionic DOPC in liposomes under the action of PLA₂.

---

KEY WORDS: pancreatic phospholipase A₂, phosphatidylethanol, phosphatidylcholine, ethanol

---