Luminol-Dependent Chemiluminescence Analysis of Chitooligosaccharide-Induced Rapid Production of Hydrogen Peroxide by Intact Wheat Seedlings

R. M. Khairullin^* and I. E. Akhmetova

Institute of Biochemistry and Genetics, Ufa Scientific Center, Russian Academy of Sciences, pr. Oktyabrya 69, Ufa, 450054 Russia; fax: (3472) 35-6100; E-mail: phyto@anrb.ru

^* To whom correspondence should be addressed.

Received April 20, 2000; Revision received November 16, 2000

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The feasibility of a luminol-dependent chemiluminescence analysis of hydrogen peroxide production by intact wheat seedlings using a KhL-003 chemiluminometer was determined. It was shown that the minimal H₂O₂ concentration that can be detected in a 0.5-ml sample with this instrument is 0.125 µM. Analysis of biological activity of a mixture of chitooligosaccharides with molecular masses from 5 to 10 kD and acetylation degree of 65% demonstrated that, at a concentration of 1 µg/ml, they induce rapid overproduction of H₂O₂ in roots of 3-day-old wheat seedlings.

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KEY WORDS: hydrogen peroxide, chemiluminescence analysis, wheat seedlings, chitooligosaccharides

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