Comparative Study of Lipid Composition and Proliferative Activity of Rat Cholangiocarcinoma RS1 and Sarcoma M1 Depending on the Transplantation Organ

V. A. Kobliakov¹, O. G. Somova², A. G. Kandyba², V. F. Kondalenko¹, N. M. Klim¹, and E. V. Dyatlovitskaya^2*

¹Institute of Carcinogenesis, Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Kashirskoe Shosse 24, Moscow, 115478 Russia

²Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997 Russia; E-mail: dyatl@ibch.ru

^* To whom correspondence should be addressed.

Received October 22, 2001; Revision received October 31, 2001

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The proliferative activity and lipid composition (phospholipids, gangliosides) were studied in rat cholangiocarcinoma RS1 and sarcoma M1 transplanted subcutaneously or intrahepatically. The mitotic index was higher in the tumors transplanted into the heterologous organ. The total phospholipid and sphingomyelin contents were higher in the tumors transplanted intrahepatically. GM3 and GD3 were the main gangliosides in both variants of each tumor. A significant amount of GM3 ganglioside lactone was found in the intrahepatic variants whereas it was absent in the subcutaneous tumors. Both the mitotic index and lipid composition of the tumors studied depended on their microenvironment.

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KEY WORDS: gangliosides, ganglioside lactone, microenvironment, tumor, proliferation, phospholipids

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