2Scientific-Production Complex Biotechindustry, 2-i Spaso-Nalivkovsky Pereulok 6, Moscow, 119991 Russia
3Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119899 Russia; fax: (095) 939-3181; E-mail: firstname.lastname@example.org
* To whom correspondence should be addressed.
Received November 8, 2001; Revision received December 17, 2001
In aqueous medium etiolated wheat seedlings release superoxide anion (O2·-). Interaction of a synthetic antioxidant, butylated hydroxytoluene (BHT, ionol), with oxygen in the aqueous medium is accompanied by O2·- formation. This suggests that under certain conditions BHT behaves as a prooxidant. A natural antioxidant, superoxide dismutase (SOD), and also a wound healing preparation, emulsified denatured placenta (EDP), do not exhibit the prooxidant properties. In contrast to BHT, they reduce O2·- production by the etiolated wheat seedling system.
KEY WORDS: BHT, antioxidant, prooxidant, wheat seedlings, reactive oxygen species, superoxide