Specificity of the Action of Lysoamidase on Staphylococcus aureus 209P Cell Walls

E. A. Begunova^*, O. A. Stepnaya, V. Ya. Lysanskaya, and I. S. Kulaev

Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142292, Moscow Region, Russia; fax: (095) 956-3370

^* To whom correspondence should be addressed.

Received April 22, 2002

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Specificity of Staphylococcus aureus 209P cell wall hydrolysis by the L1 and L2-bacteriolytic enzymes from lysoamidase lytic complex was studied. L1-peptidase was shown to display both glycyl-glycine endopeptidase and N-acetylmuramyl-L-alanine amidase enzymatic activities on the S. aureus peptidoglycan molecule, whereas L2-peptidase acts as N-acetylmuramyl-L-alanine amidase.

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KEY WORDS: lysoamidase, bacteriolytic enzymes, cell wall, substrate specificity

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