Recombinant Endo-beta-1,4-xylanase from Penicillium canescens

O. A. Sinitsyna^1*, A. V. Gusakov¹, O. N. Okunev², V. A. Serebryany³, E. A. Vavilova³, Yu. P. Vinetsky³, and A. P. Sinitsyn¹

¹Faculty of Chemistry, Lomonosov Moscow State University, Moscow 199899, Russia; fax: (7-095) 939-0997; E-mail: oasinitsyna@enzyme.chem.msu.ru

²Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142292, Moscow Region, Russia; fax: (7-095) 923-3602

³Institute of Genetics, Moscow 113545, Russia

^* To whom correspondence should be addressed.

Received March 24, 2003; Revision received April 21, 2003

---

Recombinant endo-beta-1,4-xylanase (Xyl-31_rec, 31 kD, pI 8.2-9.3, the tenth family of glycosyl hydrolases) was isolated from the culture liquid of Penicillium canescens (strain with the amplified homologous xylanase gene) by chromatofocusing on Mono P and hydrophobic chromatography on phenyl-Superose. It is shown that the biochemical and kinetic parameters, substrate specificity, stability, and other properties of the recombinant and native enzymes are almost the same. It was found that Xyl-31_rec can be used for biobleaching of cellulose, the recombinant P. canescens strains providing a high yield of extracellular Xyl-31_rec (up to 800-900 U/ml of culture liquid) and not secreting cellulases.

---

KEY WORDS: endo-beta-1,4-xylanase, chromatofocusing, hydrophobic chromatography, recombinant strain, biobleaching of cellulose

---