The second section consists of 8 chapters, where approaches to production of proteins, crystallization of membrane proteins, study of protein structures by means of X-ray analysis and NMR-spectroscopy are discussed. Some chapters describe methods for modification of protein structures, including the secondary structure.
The third section containing 6 chapters considers methods of identification of possible errors during design of models of protein tertiary structure. This section also includes description of principles of evolutionary classification of protein structures and the use of data on protein structure for drug design based on structure of biologically active proteins such as serine proteases, kinases, phosphatases, and their inhibitors. A special chapter of this section introduces the European Institute of Bioinformatics (UK, web-site http://www.ebi.ac.uk), founded in 1995. This institute has a huge database of protein structures.
The fourth section deals with some problems related to use of structural data for drug design. Particularly, it considers dependence of interferon activity on its structure and mechanisms of action of G-protein coupled receptor family and their structural relationship with rhodopsin.
This book will be useful for biochemists and specialists in bioorganic chemistry and protein structure and also for biotechnologists and pharmacologists involved in protein-structure-based drug design.
Doctor of Biological Sciences
G. Ya. Wiederschain