Extracellular Oxidases of the Lignin-Degrading Fungus Panus tigrinus

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D. A. Cadimaliev^1*, V. V. Revin¹, N. A. Atykyan¹, and V. D. Samuilov²

¹Department of Biotechnology, Biological Faculty, Ogarev Mordovian State University, Bolshevistskaya ul. 68, 430000 Saransk, Russia; fax: (8342) 32-2523; E-mail: biotech@moris.ru

²Department of Physiology of Microorganisms, Biological Faculty, Lomonosov Moscow State University, 119899 Moscow, Russia; fax: (7-095) 939-3807; E-mail: vds@8.cellimm.bio.msu.ru

^* To whom correspondence should be addressed.

Received March 30, 2004; Revision received May 21, 2004

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Two extracellular oxidases (laccases) were isolated from the extracellular fluid of the fungus Panus (Lentinus) tigrinus cultivated in low-nitrogen medium supplemented with birch sawdust. The enzymes were purified by successive chromatography on columns with TEAE-cellulose and DEAE-Toyopearl 650M. Both oxidases catalyze oxidation of pyrocatechol and ABTS. Moreover, oxidase 1 also catalyzes oxidation of guaiacol, o-phenylenediamine, and syringaldazine. The enzymes have identical pH (7.0) and temperature (60-65°C) optimums. Absorption spectra of the oxidases differ from the spectra of typical “blue” laccases and are similar to the spectrum of yellow oxidase.

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KEY WORDS: lignin, biodegradation, oxidase, laccase, white rot fungi, Panus (Lentinus) tigrinus

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