Binding Sites for Transcription Factor SF-1 in Promoter Regions of Genes Encoding Mouse Steroidogenesis Enzymes 3betaHSDI and P450c17

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T. V. Busygina^*, G. V. Vasiliev, N. V. Klimova, E. V. Ignatieva, and A. V. Osadchuk

Institute of Cytology and Genetics, Siberian Division, Russian Academy of Sciences, pr. Lavrent'eva 10, 630090 Novosibirsk, Russia; E-mail: tbusig@bionet.nsc.ru

^* To whom correspondence should be addressed.

Received August 6, 2004; Revision received October 29, 2004

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Using gel retardation of DNA samples and specific antibodies, binding sites for the transcription factor SF-1 were found in positions -53/-44 and -285/-270 in the promoter region of the mouse Cyp17 gene and in position -117/-108 of the promoter region of the mouse 3betaHSDI gene.

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KEY WORDS: SF-1 (steroidogenic factor 1), steroidogenesis enzymes, Cyp17, 3betaHSD, binding sites for SF-1, gel retardation of DNA

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