Expression of Chlamydia trachomatis Inclusion Membrane Protein Genes IncB and IncC in Escherichia coli

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E. S. Kostrjukova^*, V. N. Lazarev, G. A. Titova, T. A. Akopian, S. A. Levitskii, and V. M. Govorun

Research Institute for Physico-Chemical Medicine, Ministry of Health and Social Development of the Russian Federation, ul. Malaya Pirogovskaya 1a, 119992 Moscow, Russia; fax: (7-495) 246-4501; E-mail: eles@newmail.ru

^* To whom correspondence should be addressed.

Received August 16, 2005; Revision received September 23, 2005

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In this study, we have cloned the Chlamydia trachomatis genes incB and incC into the expression plasmid vectors from pET series for the subsequent isolation of recombinant proteins. As a result, we have obtained the first full-length recombinant C. trachomatis proteins IncB and IncC, which can be used for following antibody production and for study of their protein-protein interaction.

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KEY WORDS: Chlamydia trachomatis, inclusion membrane protein, gene polymorphism, gene expression

DOI: 10.1134/S0006297906030059

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