Two-Dimensional Electrophoretic Proteome Study of Serum Thermostable Fraction from Patients with Various Tumor Conditions

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E. I. Goufman¹, S. A. Moshkovskii^1*, O. V. Tikhonova¹, P. G. Lokhov¹, V. G. Zgoda¹, M. V. Serebryakova¹, I. Yu. Toropygin¹, M. A. Vlasova¹, M. R. Safarova², O. V. Makarov², and A. I. Archakov¹

¹Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, ul. Pogodinskaya 10, 119121 Moscow, Russia; fax: (495) 245-0857; E-mail: sergei.moshkovskii@ibmc.msk.ru

²Russian State Medical University, ul. Ostrovityanova 1, 117997 Moscow, Russia

^* To whom correspondence should be addressed.

Received August 31, 2005

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One of the problems of plasma proteomics is a presence of large major components. In this work, we use the thermostable fraction as a way to deplete these major proteins. The thermostable fraction of serum samples from patients with ovarian, uterus, and breast cancers and benign ovarian tumor was analyzed using two-dimensional electrophoresis combined with MALDI-TOF(-TOF)-mass spectrometry. Of them, alpha-1-acid glycoprotein and clusterin are expressly down-regulated in breast cancer, whereas transthyretin is decreased specifically in ovarian cancer. Apolipoprotein A-I forms have decreased spot volumes, while haptoglobin alpha1, in contrast, is elevated in several tumors. These data are partly consistent with previous art studies on cancer proteomics, which involve mass-spectrometry-based serum profiling techniques. Serum thermostable fraction may be recommended as a good tool for medium and small protein proteome investigation, in particular, by 2D-electrophoresis.

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KEY WORDS: serum thermostable fraction, ovarian cancer, breast cancer, two-dimensional (2D) electrophoresis, proteomics

DOI: 10.1134/S000629790604002X

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