Mechanism of Action of DNA-Hydrolyzing Antibodies to DNA from Blood of Patients with Systemic Lupus Erythematosus

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T. A. Nevzorova^*, V. G. Vinter, O. A. Konovalova, and M. Kh. Salakhov

Kazan State University, ul. Kremlevskaya 18, 420008 Kazan, Russia; E-mail: Tatyana.Nevzorova@ksu.ru

^* To whom correspondence should be addressed.

Received March 23, 2006; Revision received July 10, 2006

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Four fractions of IgG antibodies to native DNA (nDNA) were obtained from blood of patients with systemic lupus erythematosus (SLE). These antibodies displayed a thermostable DNA-hydrolyzing activity and were different in affinity for DNA-cellulose and sorption on DEAE-cellulose. DNA-hydrolyzing antibodies to nDNA are metal-dependent endonucleases, cause mainly single-strand breaks in DNA, and are active over a wide range of pH. By atomic-force microscopy, three-dimensional images of DNA complexes with DNA-hydrolyzing antibodies to nDNA were obtained with nanometer resolution, and a nonprocessive action mechanism was shown for the DNase activity of antibodies to nDNA.

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KEY WORDS: polyclonal catalytic antibodies, abzymes, systemic lupus erythematosus, IgG, hydrolysis of DNA, atomic-force microscopy

DOI: 10.1134/S0006297906110095

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