Influence of Culture Conditions and Virulence Plasmids on Expression of Immunoglobulin-Binding Proteins of Yersinia pseudotuberculosis

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G. A. Naberezhnykh^*, E. V. Sidorin, L. A. Lapshina, A. V. Reunov, and T. F. Solov'eva

Pacific Institute of Bioorganic Chemistry, Far-Eastern Division, Russian Academy of Sciences, pr. 100-letiya Vladivostoka 159, 690022 Vladivostok, Russia; fax: (4232) 314-050; E-mail: naber1953@mail.ru

^* To whom correspondence should be addressed.

Received July 19, 2006; Revision received August 4, 2006

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The influence of culture conditions and plasmids on immunoglobulin (Ig)-binding activity of two isogenic strains of Yersinia pseudotuberculosis (plasmid-free strain 48^-82^- and strain 48⁺82⁺ bearing plasmids pYV48 and pVM82) was studied. The highest activity was observed in the bacteria grown on glucose-containing liquid medium in the stationary growth phase. The Ig-binding activity of the bacteria cultured on the liquid medium at pH 6.0 was about 1.5-fold higher than that of the bacteria grown at pH 7.2. Expression of the Ig-binding proteins (IBPs) was most influenced by temperature of cultivation. The IBP biosynthesis was activated in the bacteria grown at 4°C and markedly decreased in those grown at 37°C. The Ig-binding activity of lysates from the bacteria was caused by proteins with molecular weights of 7-20 kD. The activities of the plasmid-free and plasmid-bearing Y. pseudotuberculosis strains (48^-82^- and 48⁺82⁺, respectively) were analyzed, and the plasmids were shown to have no effect on the IBP expression and biosynthesis, which seemed to be determined by chromosomal genes.

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KEY WORDS: Yersinia pseudotuberculosis, immunoglobulin-binding proteins, culture conditions, plasmids

DOI: 10.1134/S0006297906110150

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