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DNA-methyltransferase SsoII as a Bifunctional Protein: Features of the Interaction with the Promoter Region of SsoII Restriction-Modification Genes


A. S. Romanenkov1, O. V. Kisil1, T. S. Zatsepin1, O. V. Yamskova1, A. S. Karyagina2,3, V. G. Metelev1, T. S. Oretskaya1,4, and E. A. Kubareva4*

1Chemical Faculty, Lomonosov Moscow State University, 119992 Moscow, Russia

2Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, ul. Gamalei 18, 123098 Moscow, Russia

3All-Russian Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences, Timiryazevskaya ul. 42, 127550 Moscow, Russia

4Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; fax: (495) 939-3181; E-mail: kubareva@belozersky.msu.ru

* To whom correspondence should be addressed.

Received June 21, 2006; Revision received August 17, 2006
DNA duplexes bearing an aldehyde group at the 2´-position of the sugar moiety were used for affinity modification of (cytosine-5)-DNA methyltransferase SsoII. It is shown that lysine residues of M.SsoII N-terminal region are located in proximity to DNA sugar-phosphate backbone of a regulatory sequence of promoter region of SsoII restriction-modification enzyme coding genes. The ability of the two M.SsoII subunits to interact with DNA regulatory sequence has been demonstrated by affinity modification using DNA duplexes with two 2´-aldehyde groups. Changes in nucleotide sequence of one half of the regulatory region prevented cross-linking of the second M.SsoII subunit. The results on sequential affinity modification of M.SsoII by two types of modified DNA ligands (i.e. by 2´-aldehyde-containing and phosphoryldisulfide-containing) have demonstrated the possibility of covalent attachment of the protein to two different DNA recognition sites: regulatory sequence and methylation site.
KEY WORDS: (cytosine-5)-DNA methyltransferases, modified oligonucleotides, affinity modification, 2´-aldehyde group, phosphoryldisulfide group

DOI: 10.1134/S0006297906120091