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An Improved Sample Preparation Method for Analyzing Mycobacterial Proteins in Two-Dimensional Gels

D. Bisht*, N. Singhal, P. Sharma, and K. Venkatesan

Department of Biochemistry, National JALMA Institute for Leprosy and Other Mycobacterial Diseases (Indian Council of Medical Research), Tajganj, Agra-282 001, India; fax: (910562) 233-1755; E-mail: abd1109@rediffmail.com

* To whom correspondence should be addressed.

Received December 20, 2006; Revision received March 29, 2007
Two-dimensional gel electrophoresis (2-DE) is currently a widely used analytical method for resolving complex mixtures of proteins. Sample preparation has a marked influence on 2-DE pattern. To reduce impurities and to increase the low-abundance proteins, protein precipitation is often used for the preparation of samples before 2-DE. In this study, we revealed that addition of SDS prior to TCA precipitation of mycobacterial cell extract proteins increases the resolution of the 2-D gel pattern.
KEY WORDS: mycobacteria, tuberculosis, protein precipitation, two-dimensional gel electrophoresis

DOI: 10.1134/S0006297907060119