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Role of Extracellular Calcium Ions at Early Stages of Human T-Lymphocyte Polyclonal Activation in vitro

I. S. Litvinov1*, M. A. Fomichev2, and M. P. Kirpichnikov1

1Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russia; fax: (495) 330-4011; E-mail: lis@mx.ibch.ru

2Moscow State Medical Stomatological University, ul. Delegatskaya 10, 111125 Moscow, Russia

* To whom correspondence should be addressed.

Received February 4, 2008; Revision received October 8, 2008
In this work we comparatively analyzed interleukin-2 (IL-2) and interferon γ production (IFN-γ) and also CD69 and CD25 expression by activated T-cells depending on extracellular calcium concentration ([Ca2+]e), which was varied with EGTA. The expression of CD69 molecules on the surface of T-cells depended only on the presence of phorbol myristate acetate, occurred at [Ca2+]e higher than 0.2 mM, and did not require the presence of ionomycin. The increase in [Ca2+]e by itself cannot induce expression of CD25 and CD69 molecules by activated cells. The values of [Ca2+]e, at which maximal fractions of CD3+CD69+(IL-2)+, CD3+CD69+(IFN-γ)+, and CD3+CD25+ activated T-cells were reached, never coincided with mean values of [Ca2+]e for healthy donors and were different from each other. So, there is different [Ca2+]e dependence for initial stages of activated T-cells differentiation. The relation between T-cells activation parameters and their differentiation is discussed.
KEY WORDS: human peripheral blood, ionomycin, PMA, cell activation, EGTA, IL-2, IFN-γ

DOI: 10.1134/S0006297909030043