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Electrochemistry of Cytochromes P450: Analysis of Current–Voltage Characteristics of Electrodes with Immobilized Cytochromes P450 for the Screening of Substrates and Inhibitors

V. V. Shumyantseva*, T. V. Bulko, G. P. Kuznetsova, N. F. Samenkova, and A. I. Archakov

Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, ul. Pogodinskaya 10, 119121 Moscow, Russia; fax: (499) 245-0857; E-mail: viktoria.shumyantseva@ibmc.msk.ru

* To whom correspondence should be addressed.

Received February 26, 2008; Revision received March 14, 2008
In the current study, an approach to elucidating the substrate specificity of cytochromes P450 based on the analysis of current–voltage characteristics of voltammograms and amperograms is proposed. Data on the electrochemical behavior of bioelectrodes with immobilized cytochromes P450 2B4, 1A2, 3A4, 11A1 (P450scc), and 51b1 (Mycobacterium tuberculosis sterol 14α-demethylase or CYP51 MT) in the presence of typical substrates and inhibitors for these hemoprotein forms are reported. Immobilization of the enzymes was accomplished by using graphite screen-printed electrodes modified with gold nanoparticles and with the synthetic membrane-like compound didodecyldimethylammonium bromide. The method of electro-analysis can be applied to the search of potential substrates and inhibitors of cytochromes P450 and to creation of multichannel electrochemical plates (chips, panels) with immobilized cytochromes P450.
KEY WORDS: cytochrome P450, colloidal gold nanoparticles, bioelectrochemistry, drug metabolism

DOI: 10.1134/S0006297909040129