2Lomonosov Moscow State University, 119992 Moscow, Russia; fax: (495) 939-0997; E-mail: email@example.com
* To whom correspondence should be addressed.
Received December 3, 2008; Revision received January 15, 2009
Gene egl2 of secreted endo-(1–4)-β-glucanase of glycosyl hydrolase family 5 of the mycelial fungus Penicillium canescens was cloned. The gene was expressed in P. canescens under control of a strong promoter of the bgaS gene encoding β-galactosidase of P. canescens, and endoglucanase producing strains were obtained. Chromatographically purified recombinant 48 kDa protein had pH and temperature optima 3.4 and 60°C, respectively, exhibited specific activity of 33 IU, and had Km and Vmax in CM-cellulose hydrolysis of 10.28 g/liter and 0.26 µmol/sec per mg, respectively.
KEY WORDS: endoglucanase, mycelial fungus, Penicillium canescens, gene