Effect of Transient Acid Stress on the Proteome of Intestinal Probiotic Lactobacillus reuteri

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KiBeom Lee^1* and KyungBae Pi²

¹Department of Biotechnology, SongDo Techno Park, 7-50 Songdo-Dong, Yeonsu-Gu, Incheon, Korea 406-840; fax: (8232)260-0769; E-mail: klee02@empal.com

²Research Institute, AdipoGen, Room #401, Venture Building B, SongDo TechnoPark, 7-50 Songdo-Dong, Yeonsu-Gu, Incheon, Korea 406-840

^* To whom correspondence should be addressed.

Received May 13, 2009; Revision received June 3, 2009

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We report the acid tolerance response and changes in the level of protein expression of probiotic Lactobacillus reuteri subjected to transient (1.5 h) acid stress at pH 3.0. Sixteen acid-responsive proteins were identified by peptide mass fingerprinting including members of five broad functional categories: metabolism, transcription/translation, DNA replication/repair, transport and binding proteins, and pH homeostasis and stress responses. This work can provide some new and relevant information on the inducible mechanisms underlying the capacity of probiotic L. reuteri to tolerate acid stress.

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KEY WORDS: Lactobacillus reuteri, acid stress response, two-dimensional gel electrophoresis (2-DE), matrix-assisted laser-desorption-ionization time-of-flight-mass spectrometry (MALDI-TOF MS)

DOI: 10.1134/S0006297910040097

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