Structure of an Acidic O-Specific Polysaccharide of the Marine Bacterium Pseudoalteromonas agarivorans KMM 232 (R-form)

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N. A. Komandrova^1*, V. V. Isakov¹, S. V. Tomshich¹, L. A. Romanenko¹, A. V. Perepelov², and A. S. Shashkov²

¹Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, pr. 100-letiya Vladivostoka 159, 690022 Vladivostok, Russia; fax: (4232) 314-050; E-mail: komand@piboc.dvo.ru

²Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991 Moscow, Russia

^* To whom correspondence should be addressed.

Received July 9, 2009; Revision received December 11, 2009

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An acidic O-specific polysaccharide containing L-rhamnose, 2-acetamido-2-deoxy-D-galactose, 2,6-dideoxy-2-(N-acetyl-L-threonine)amino-D-galactose, and 2-acetamido-2-deoxy-D-mannuronic acid was obtained by mild acid degradation of the lipopolysaccharide of the marine bacterium Pseudoalteromonas agarivorans KMM 232 (R-form) followed by gel-permeation chromatography. The polysaccharide was subjected to Smith degradation to give a modified polysaccharide with trisaccharide repeating unit containing L-threonine. The initial and modified polysaccharides were studied by sugar analysis and ¹H- and ¹³C-NMR spectroscopy, including COSY, TOCSY, ROESY, and HSQC experiments, and the structure of the branched tetrasaccharide repeating unit of the polysaccharide was established.

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KEY WORDS: Pseudoalteromonas agarivorans, marine bacteria, bacterial polysaccharide structure, O-specific polysaccharide, NMR spectroscopy, threonine

DOI: 10.1134/S0006297910050123

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