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Synthesis of Sulfated Pectins and Their Anticoagulant Activity

F. V. Vityazev1*, V. V. Golovchenko1, O. A. Patova1, N. N. Drozd2, V. A. Makarov2, A. S. Shashkov3, and Yu. S. Ovodov1

1Institute of Physiology, Komi Science Center, Ural Branch of the Russian Academy of Sciences, ul. Pervomaiskaya 50, 167982 Syktyvkar, Russia; fax: (8212) 241-001; E-mail: ovoys@physiol.komisc.ru

2Hematological Scientific Center, Russian Academy of Medical Sciences, Novyi Zykovskii Proezd 4a, 125167 Moscow, Russia; fax: (495) 614-7611; E-mail: nndrozd@mail.ru

3Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 117913 Moscow, Russia; fax: (495) 135-5328; E-mail: shash@ioc.ac.ru

* To whom correspondence should be addressed.

Received July 3, 2009; Revision received October 13, 2009
The following pectins were sulfated: bergenan BC (the pectin of Bergenia crassifolia L), lemnan LM (the pectin of Lemna minor L), and galacturonan as a backbone of pectins. Pyridine monomethyl sulfate, pyridine sulfotrioxide, and chlorosulfonic acid were used as reagents for sulfation. Chlorosulfonic acid proved to be the optimal reagent for sulfation of galacturonan and other pectins. Galacturonan and pectin derivatives with different degrees of sulfation were synthesized and their anticoagulant activities were shown to depend on the quantity of sulfate groups in the pectin macromolecules.
KEY WORDS: lemnan, bergenan, sulfated pectin, sulfated galacturonan, anticoagulant activity, Bergenia crassifolia L, Lemna minor L

DOI: 10.1134/S0006297910060118