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Lipids of Nuclear Fractions from Neurons and Glia of Rat Neocortex under Conditions of Artificial Hypobiosis

I. K. Kolomiytseva*, L. N. Markevich, D. A. Ignat’ev, and O. V. Bykova

Institute of Cell Biophysics, Russian Academy of Sciences, ul. Institutskaya 3, 142290 Pushchino, Moscow Region, Russia; fax: (4967) 330-509; E-mail: kolomiytseviskra@rambler.ru

* To whom correspondence should be addressed.

Received August 7, 2009; Revision received December 15, 2009
Lipid contents were studied in tissue and nuclei isolated from neurons and glia of neocortex of rats under conditions of normothermia and in the state of artificial hypobiosis caused by hypothermia–hypoxia–hypercapnia. Compared to the neocortex tissue, both nuclear fractions were fivefold impoverished in phospholipids and cholesterol and strongly enriched with mono- and diglycerides and fatty acids. The nuclear fractions from neurons and glia contained similar amounts of phospholipids, and only the cardiolipin content in the neuronal nuclei was lower than in the glial nuclei. The state of artificial hypobiosis in rats led to an increase in the cholesterol/phospholipids ratio (mol/mol) in the nuclei from the neurons and glia; amounts of cholesterol and sphingomyelin in the nuclei from the glia were increased. The increases in the cholesterol and sphingomyelin contents and in the cholesterol/phospholipids ratio suggest an involvement of lipid-dependent signaling systems of the nuclei in the functional response of mammalian neocortex cells to artificial hypobiosis.
KEY WORDS: lipids, rats, hypobiosis, nuclei, neurons, glia

DOI: 10.1134/S0006297910090063