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Foot and Mouth Disease Virus Polyepitope Protein Produced in Bacteria and Plants Induces Protective Immunity in Guinea Pigs

E. P. Andrianova1, S. R. Krementsugskaia2, N. N. Lugovskaia2, T. K. Mayorova2, V. V. Borisov2, M. A. Eldarov1, N. V. Ravin1, A. S. Folimonov1*, and K. G. Skryabin1

1Bioengineering Center, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7-1, 117312 Moscow, Russia; fax: (499) 135-0571; E-mail: folimonov@biengi.ac.ru

2Federal State Institution “Federal Center for Animal Health”, Yuryevets, 600901 Vladimir, Russia; fax: (492) 226-1573; E-mail: borisov2@arriah.ru

* To whom correspondence should be addressed.

Received September 20, 2010; Revision received November 8, 2010
The goal of this project was to develop an alternative foot and mouth disease (FMD) vaccine candidate based on a recombinant protein consisting of efficient viral epitopes. A recombinant gene was designed that encodes B-cell epitopes of proteins VP1 and VP4 and T-cell epitopes of proteins 2C and 3D. The polyepitope protein (H-PE) was produced in E. coli bacteria or in N. benthamiana plants using a phytovirus expression system. The methods of extraction and purification of H-PE proteins from bacteria and plants were developed. Immunization of guinea pigs with the purified H-PE proteins induced an efficient immune response against foot and mouth disease virus (FMDV) serotype O/Taiwan/99 and protection against the disease. The polyepitope protein H-PE can be used as a basis for developing a new recombinant vaccine against FMD.
KEY WORDS: FMDV, vaccine, transient expression in plants, polyepitope protein

DOI: 10.1134/S0006297911030072