2Federal State Institution “Federal Center for Animal Health”, Yuryevets, 600901 Vladimir, Russia; fax: (492) 226-1573; E-mail: email@example.com
* To whom correspondence should be addressed.
Received September 20, 2010; Revision received November 8, 2010
The goal of this project was to develop an alternative foot and mouth disease (FMD) vaccine candidate based on a recombinant protein consisting of efficient viral epitopes. A recombinant gene was designed that encodes B-cell epitopes of proteins VP1 and VP4 and T-cell epitopes of proteins 2C and 3D. The polyepitope protein (H-PE) was produced in E. coli bacteria or in N. benthamiana plants using a phytovirus expression system. The methods of extraction and purification of H-PE proteins from bacteria and plants were developed. Immunization of guinea pigs with the purified H-PE proteins induced an efficient immune response against foot and mouth disease virus (FMDV) serotype O/Taiwan/99 and protection against the disease. The polyepitope protein H-PE can be used as a basis for developing a new recombinant vaccine against FMD.
KEY WORDS: FMDV, vaccine, transient expression in plants, polyepitope protein