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Major Chondroitin Sulfate Proteoglycans Identified in L6J1 Myoblast Culture

I. I. Ermakova1,2*, G. A. Sakuta1, M. A. Potekhina1, M. A. Fedorova3, R. Hoffmann3, and V. I. Morozov1,4

1Department of Cell Cultures, Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr. 4, 194064 St. Petersburg, Russia; fax: (812) 297-0341; E-mail: irinabiochem@mail.ru

2Pavlov Institute of Physiology, Russian Academy of Sciences, Makarova emb. 6, 199034 St. Petersburg, Russia; fax: (812) 328-0501; E-mail: tch@infran.ru

3Institute of Bioanalytical Chemistry, Center for Biotechnology and Biomedicine, Faculty of Chemistry and Mineralogy, Leipzig University, Deutscher Platz 5, 04103 Leipzig, Germany

4Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, pr. Toreza 44, 194223 St. Petersburg, Russia; fax: (812) 552-3012; E-mail: office@iephb.ru

* To whom correspondence should be addressed.

Received August 2, 2010; Revision received October 19, 2010
The major proteoglycans from L6J1 rat myoblast culture were identified. The proteoglycans were isolated from different constituents of cell culture: culture medium, extracellular matrix (ECM), and myoblasts. To identify their core proteins, the proteoglycans were treated with enzymes specifically digesting chondroitin/dermatan sulfates or chondroitin sulfates. Subsequent electrophoresis and mass spectrometry revealed versican, collagen XII, and inter-α-trypsin inhibitor classified as chondroitin sulfate proteoglycans and biglycan known to be chondroitin/dermatan sulfate proteoglycan. Versican was identified in ECM and the other proteoglycans in the culture medium. Such difference in localization is likely to be a consequence of different biological functions. Versican, collagen XII, and biglycan are synthesized by myoblasts and inter-α-trypsin inhibitor originates from fetal bovine serum (a culture medium component).
KEY WORDS: proteoglycans, glycosaminoglycans, myoblasts

DOI: 10.1134/S0006297911030102