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Correlation between Influence of Polysaccharides on Hydrolase Activity and Their Antiviral Effect in Tobacco Leaves

L. A. Lapshina, V. P. Nagorskaya, A. V. Reunov*, A. O. Barabanova, N. M. Shevchenko, I. M. Yermak, T. N. Zvyagintseva, and L. A. Elyakova

Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, pr. 100 let Vladivostoku 159, 690022 Vladivostok, Russia; fax: (4232) 314-050; E-mail: antreunov@mail.ru

* To whom correspondence should be addressed.

Received October 21, 2010; Revision received November 22, 2010
The activities of hydrolases (acid phosphatase, RNase, and proteases) in healthy and tobacco mosaic virus-infected leaves of Nicotiana tabacum L. var. Samsun, both untreated and treated with polysaccharides (PS) (1,3;1,6-β-D-glucan, fucoidan, and κ/β-carrageenan), were determined. The PS lead to substantial increase in the hydrolase level. The percentage of viral particles undergoing destructive change also increases in leaves treated with PS 24 h before infection. We suppose that the PS-mediated hydrolase activation promotes intracellular destruction of the viral particles and, thus, comprises one of the PS-induced protective mechanisms limiting intracellular viral accumulation.
KEY WORDS: 1,3;1,6-β-D-glucan, fucoidan, κ/β-carrageenan, acid phosphatase, RNase, protease, tobacco mosaic virus

DOI: 10.1134/S0006297911040092