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Cyanines as Efficient Photosensitizers in Photodynamic Reaction: Photophysical Properties and in vitro Photodynamic Activity

J. Kulbacka1*, A. Pola2, D. Mosiadz2, A. Choromanska1, P. Nowak3, M. Kotulska4, M. Majkowski5, A. Hryniewicz-Jankowska5, L. Purzyc1, and J. Saczko1

1Medical University, Department of Medical Biochemistry, Chalubinskiego 10, 50-368 Wroclaw, Poland; fax: +4871-784-0085; E-mail: jkulbacka@gmail.com

2Medical University, Department of Biophysics, Chalubinskiego 10, 50-368 Wroclaw, Poland

3Wroclaw University of Technology, Institute of Physical and Theoretical Chemistry, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland

4Wroclaw University of Technology, Institute of Biomedical Engineering and Instrumentation, Wybrzeze Wyspianskiego 27, office D-1/115, Wroclaw, Poland

5Wroclaw University, Institute of Biochemistry and Molecular Biology, Laboratory of Cytobiochemistry, Tamka 2, 50-137 Wroclaw, Poland

* To whom correspondence should be addressed.

Received October 28, 2010; Revision received November 26, 2010
The purpose of the present study was to explore the potential application of cyanines in photodynamic treatment. The photophysical features of four cyanines (KF570, HM118, FBF-749, and ER-139) were investigated by elemental and spectral analyses. Two malignant cell lines (MCF-7/WT and MCF-7/DOX) were used to test the potential for use in the photodynamic therapy. The cytotoxic effects of these dyes were determined by the MTT assay after 4 and 24 h of incubation with the cyanine. KF570 and HM118 were irradiated with red light (630-nm filter) and FBF-749 and ER-139 with green light (435-nm filter). The results showed that the cyanine HM118 demonstrated a major phototoxic effect. It was also noted that the efficiency of photodynamic therapy was higher in the doxorubicin-resistant cell line (MCF-7/DOX).
KEY WORDS: breast adenocarcinoma cells, cyanines, photodynamic therapy, cytotoxicity

DOI: 10.1134/S0006297911040110