2Department of Cardiothoracic Surgery, New York University School of Medicine, Bellevue Hospital, 462 First Avenue, Room 15N 20-22, 10016 New York, NY, USA; E-mail: firstname.lastname@example.org
3Centre for Biological Safety (ZBS2), Robert Koch Institute, Nordufer 20, D-13353 Berlin, Germany; E-mail: grunowR@rki.de
4Institute of Cellular and Molecular Medicine, Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen, Denmark; E-mail: email@example.com
* To whom correspondence should be addressed.
Received January 31, 2011; Revision received February 14, 2011
The repertoire of natural anti-glycan antibodies in naïve chickens and in chickens immunized with bacteria Burkholderia mallei, Burkholderia pseudomallei, and Francisella tularensis as well as with peptides from an outer membrane protein of B. pseudomallei was studied. A relatively restricted pattern of natural antibodies (first of all IgY against bacterial cell wall peptidoglycan fragments, L-Rha, and core N-acetyllactosamine) shrank and, moreover, the level of detectable antibodies decreased as a result of immunization.
KEY WORDS: bacterial polysaccharides, natural antibodies, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis