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Interaction of Transketolase from Human Tissues with Substrates

L. E. Meshalkina*, O. N. Solovjeva, and G. A. Kochetov

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; fax: (495) 939-3181; E-mail: luda@genebee.msu.ru

* To whom correspondence should be addressed.

Received January 12, 2011; Revision received April 1, 2011
The Michaelis constant values for substrates of transketolase from human tissues were determined over a wide range of substrate concentrations. It is shown that Km values determined by other authors are significantly overestimated and explained why this is so.
KEY WORDS: human tissues transketolase, thiamine diphosphate, Km for substrates of human tissues transketolase

DOI: 10.1134/S0006297911090112