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Structure of the O-Specific Polysaccharide of the Marine Bacterium Arenibacter palladensis KMM 3961T Containing 2-Acetamido-2-deoxy-L-galacturonic Acid

S. V. Tomshich*, V. V. Isakov, N. A. Komandrova, and L. S. Shevchenko

Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, pr. 100-let Vladivostoku 159, 690022 Vladivostok, Russia; fax: (423) 231-4050; E-mail: tomsh@piboc.dvo.ru

* To whom correspondence should be addressed.

The O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the marine bacterium Arenibacter palladensis type strain KMM 3961T and studied by chemical methods and 1H and 13C NMR spectroscopy including 2D COSY, TOCSY, 1H,13C HSQC, and HMBC experiments. The polysaccharide was shown to consist of tetrasaccharide repeating units containing two mannose residues (Man), one 2-acetamido-2-deoxy-D-galactose residue (D-GalNAc), and one 2-acetamido-2-deoxy-L-galacturonic acid residue (L-GalNAcA) and having the following structure:


KEY WORDS: Arenibacter palladensis, marine bacteria, O-specific polysaccharide, structure, NMR spectroscopy, 2-acetamido-2-deoxy-L-galacturonic acid

DOI: 10.1134/S0006297912010105