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Nitric Oxide – an Activating Factor of Adenosine Deaminase 2 in vitro

Ye. G. Sargisova*, N. A. Andreasyan, H. L. Hayrapetyan, and H. A. Harutyunyan

Bunyatyan Institute of Biochemistry, National Academy of Sciences of the Republic of Armenia, ul. P. Sevak 5/1, Yerevan 0014, Republic of Armenia; fax: (37410) 297-343; E-mail: biochem@ipia.sci.am

* To whom correspondence should be addressed.

Received April 21, 2011; Revision received August 31, 2011
In this study we have investigated the effect of reactive oxygen species produced by some chemicals in aqueous solutions on activity of adenosine deaminase 2 (ADA2) purified from human blood plasma. An activating effect on ADA2 was observed in vitro with sodium nitroprusside (SNP), the source of NO (nitrosonium ions NO in aqueous solutions). Not SH-groups of cysteine but other amino acid residues sensitive to NO were responsible for ADA2 activation. The SNP-derived activation was more pronounced when purified ADA2 was preincubated with heparin and different proteins as an experimental model of the protein environment in vivo. The most effective was heparin, which is known for its ability to regulate enzyme and protein functions in extracellular matrix. We conclude that ADA2 is a protein with flexible conformation that is affected by the protein environment, and it changes its activity under oxidative (nitrosative) stress.
KEY WORDS: adenosine deaminase 2, enzyme activation, NO, conformation, allostery, heparin

DOI: 10.1134/S0006297912010117