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Effect of Potential-Dependent Potassium Uptake on Calcium Accumulation in Rat Brain Mitochondria

O. V. Akopova*, L. I. Kolchinskaya, V. I. Nosar, V. A. Bouryi, I. N. Mankovskaya, and V. F. Sagach

Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, ul. Bogomoltsa 4, Kiev-24, Ukraine; E-mail: a-dubensky@mail.ru

* To whom correspondence should be addressed.

Received June 13, 2012; Revision received September 19, 2012
The effect of potential-dependent potassium uptake at 0-120 mM K+ on matrix Ca2+ accumulation in rat brain mitochondria was studied. An increase in oxygen consumption and proton extrusion rates as well as increase in matrix pH with increase in K+ content in the medium was observed due to K+ uptake into the mitochondria. The accumulation of Ca2+ was shown to depend on K+ concentration in the medium. At K+ concentration ≤30 mM, Ca2+ uptake is decreased due to K+-induced membrane depolarization, whereas at higher K+ concentrations, up to 120 mM K+, Ca2+ uptake is increased in spite of membrane depolarization caused by matrix alkalization due to K+ uptake. Mitochondrial K+ATP-channel blockers (glibenclamide and 5-hydroxydecanoic acid) diminish K+ uptake as well as K+-induced depolarization and matrix alkalization, which results in attenuation of the potassium-induced effects on matrix Ca2+ uptake, i.e. increase in Ca2+ uptake at low K+ content in the medium due to the smaller membrane depolarization and decrease in Ca2+ uptake at high potassium concentrations because of restricted rise in matrix pH. The results show the importance of potential-dependent potassium uptake, and especially the K+ATP channel, in the regulation of calcium accumulation in rat brain mitochondria.
KEY WORDS: brain, mitochondria, Ca2+ uptake, K+ uptake, K+ATP channel

DOI: 10.1134/S0006297913010100