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Influence of the Fungus Trichoderma harzianum on the Enzyme and Polysaccharide Composition of Silene vulgaris Callus

E. A. Günter*, O. M. Kapustina, and Yu. S. Ovodov

Institute of Physiology, Komi Research Center, Ural Branch of the Russian Academy of Sciences, ul. Pervomaiskaya 50, 167982 Syktyvkar, Russia; fax: (8212) 241-001; E-mail: gunter@physiol.komisc.ru

* To whom correspondence should be addressed.

Received July 10, 2012; Revision received October 29, 2012
Activities of polygalacturonase and 1,3-β-glucanase increased in campion (Silene vulgaris) callus cells during co-cultivation with the fungus Trichoderma harzianum. This was associated with a decrease in galacturonic acid residues in the pectic polysaccharide of campion silenan and also in the production of pectin by the callus. Co-cultivation of the callus and the fungus resulted in an increase in contents of arabinose residues in the intracellular arabinogalactan and in contents of galactose residues in the extracellular arabinogalactan.
KEY WORDS: callus, Trichoderma harzianum, Silene vulgaris, enzymes, pectic polysaccharides, silenan

DOI: 10.1134/S0006297913030115