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Targeted Delivery of siRNA to Differentiated Murine Myotubes in Culture by a Conjugate of Cationic Oligopeptide with FS2 Venom

V. A. Furalyov*, I. V. Kravchenko, and V. O. Popov

Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, 119071 Moscow, Russia; fax: (495) 954-2732; E-mail: vpopov@inbi.ras.ru

* To whom correspondence should be addressed.

Received September 30, 2012; Revision received December 5, 2012
A conjugate of the ligand of FS2 venom dihydropyridine receptors with a cationic arginine-containing oligopeptide was synthesized. It was found that the conjugate provides siRNA delivery to murine myotubes differentiated in vitro. The effect of RNA interference with the use of siRNA complexes with the conjugate was observed when siRNA concentrations were an order of magnitude lower than those used in the case of siRNA complexes with a non-conjugated oligopeptide.
KEY WORDS: siRNA, RNA interference, myostatin, myotubes

DOI: 10.1134/S0006297913040111