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Expression of Chicken CTCF Gene in COS-1 Cells and Partial Purification of CTCF Protein

E. S. Kotova, I. V. Sorokina, S. B. Akopov*, L. G. Nikolaev, and E. D. Sverdlov

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russia; fax: (495) 330-6538; E-mail: akser@ibch.ru

* To whom correspondence should be addressed.

Received April 12, 2013
The chicken gene for transcription factor CTCF was expressed in COS-1 mammalian cells. The CTCF protein containing polyhistidine tag was partially purified using metallo-affinity and ion-exchange chromatography. The expressed protein localized in the cell nucleus and was shown to be functionally active in the electrophoretic mobility shift assay and specifically interacted with anti-CTCF antibodies.
KEY WORDS: transcription factor CTCF, expression in mammalian cells, COS-1

DOI: 10.1134/S0006297913080038