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Isolation and Properties of Flight Muscle Mitochondria of the Bumblebee Bombus terrestris (L.)

M. Yu. Syromyatnikov1, A. V. Lopatin1, A. A. Starkov2*, and V. N. Popov1

1Voronezh State University, Universitetskaya pl. 1, 394006 Voronezh, Russia; fax: +7 (473) 220-8755; E-mail: syromyatnikov@bio.vsu.ru

2Weill Medical College Cornell University, New York, 525 East 68th Street, A501, NY 10065, USA; fax: 011 (212) 746-8276; E-mail: ans2024@med.cornell.edu

* To whom correspondence should be addressed.

Received January 9, 2013; Revision received April 11, 2013
This report describes the isolation procedure and properties of tightly coupled flight muscle mitochondria of the bumblebee Bombus terrestris (L.). The highest respiratory control index was observed upon oxidation of pyruvate, whereas the highest respiration rates were registered upon oxidation of a combination of the following substrates: pyruvate + malate, pyruvate + proline, or pyruvate + glutamate. The respiration rates upon oxidation of malate, glutamate, glutamate + malate, or succinate were very low. At variance with flight muscle mitochondria of a number of other insects reported earlier, B. terrestris mitochondria did not show high rates of respiration supported by oxidation of proline. The maximal respiration rates were observed upon oxidation of α-glycerophosphate. Bumblebee mitochondria are capable of maintaining high membrane potential in the absence of added respiratory substrates, which was completely dissipated by the addition of rotenone, suggesting high amount of intramitochondrial NAD-linked oxidative substrates. Pyruvate and α-glycerophosphate appear to be the optimal oxidative substrates for maintaining the high rates of oxidative metabolism of the bumblebee mitochondria.
KEY WORDS: insects, bumblebee, flight muscle, mitochondria, oxidative substrates, oxidative phosphorylation

DOI: 10.1134/S0006297913080075