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REVIEW: Recombinant Horseradish Peroxidase: Production and Analytical Applications

V. G. Grigorenko1*, I. P. Andreeva1, M. Yu. Rubtsova1, and A. M. Egorov1,2

1Lomonosov Moscow State University, Chemical Faculty, 119991 Moscow, Russia; fax: +7 (495) 939-2742; E-mail: vitaly.grigorenko@gmail.com

2Russian Medical Academy of Post-Graduate Education, Microbiology Department, 125993 Moscow, Russia

* To whom correspondence should be addressed.

Received November 14, 2014; Revision received December 9, 2014
Horseradish peroxidase is a key enzyme in bio- and immunochemical analysis. New approaches in functional expression of the peroxidase gene in E. coli cells and the subsequent refolding of the resulting protein yield a recombinant enzyme that is comparable in its spectral and catalytic characteristics to the native plant peroxidase. Genetic engineering approaches allow production of recombinant peroxidase conjugates with both protein antigens and Fab antibody fragments. The present article reviews the use of recombinant horseradish peroxidase as the marker enzyme in ELISA procedures as well as in amperometric sensors based on direct electron transfer.
KEY WORDS: horseradish peroxidase, recombinant conjugate, amperometric biosensor

DOI: 10.1134/S0006297915040033