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REVIEW: Detection of Intermolecular Interactions Based on Surface Plasmon Resonance Registration

D. V. Sotnikov, A. V. Zherdev, and B. B. Dzantiev*

Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia; E-mail: sotnikov-d-i@mail.ru; zherdev@inbi.ras.ru; dzantiev@inbi.ras.ru

* To whom correspondence should be addressed.

Received September 16, 2015
Methods for registration of intermolecular interactions based on the phenomenon of surface plasmon resonance (SPR) have become one of the most efficient tools to solve fundamental and applied problems of analytical biochemistry. Nevertheless, capabilities of these methods are often insufficient to detect low concentrations of analytes or to screen large numbers of objects. That is why considerable efforts are directed at enhancing the sensitivity and efficiency of SPR-based measurements. This review describes the basic principles of the detection of intermolecular interactions using this method, provides a comparison of various types of SPR detectors, and classifies modern approaches to enhance sensitivity and efficiency of measurements.
KEY WORDS: surface plasmon resonance, registration of intermolecular interactions, enhancement of analytical signal, multiparametric assay

DOI: 10.1134/S0006297915130131