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Features of Gene Expression of Bacillus pumilus Metalloendopeptidase

N. L. Rudakova*, A. R. Sabirova, N. P. Balaban, A. O. Tikhonova, and M. R. Sharipova

Kazan (Volga Region) Federal University, 420008 Kazan, Russia; E-mail: natalialrudakova@mail.ru

* To whom correspondence should be addressed.

Received April 19, 2016; Revision received May 20, 2016
Features of gene expression of the secreted Bacillus pumilus metalloendopeptidase belonging to the adamalysin/reprolysin family were investigated. In the regulatory region of the gene, we identified hypothetical binding sites for transcription factors CcpA and TnrA. We found that the expression of the metalloendopeptidase gene is controlled by mechanisms of carbon and nitrogen catabolite repression. In experiments involving nitrogen metabolism regulatory protein mutant strains, we found that the control of the metalloendopeptidase gene expression involves proteins of ammonium transport GlnK and AmtB interacting with the TnrA-regulator.
KEY WORDS: Bacillus pumilus, metalloendopeptidase, gene expression, catabolite repression, regulatory mutants

DOI: 10.1134/S0006297916080095