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Cell Wall Glycopolymers of Type Strains from Three Species of the Genus Actinoplanes

G. M. Streshinskaya1*, A. S. Sashkov2, E. M. Tul’skaya1, S. N. Senchenkova2, A. S. Dmitrenok2, N. F. Piskunkova1, O. V. Bueva3, and L. I. Evtushenko3

1Lomonosov Moscow State University, Faculty of Biology, 119991 Moscow, Russia; fax: +7 (495) 939-4309; E-mail: streshinskaya@mail.ru

2Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia; fax: +7 (499) 135-5328

3All-Russian Collection of Microorganisms, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia; fax: +7 (496) 956-3370

* To whom correspondence should be addressed.

Received May 18, 2016; Revision received June 23, 2016
The structures of cell wall glycopolymers from the type strains of three Actinoplanes species were investigated using chemical methods, NMR spectroscopy, and mass spectrometry. Actinoplanes digitatis VKM Ac-649T contains two phosphate-containing glycopolymers: poly(diglycosyl-1-phosphate) →6)-α-D-GlcpNAc-(1-P-6)-α-D-GlcpN-(1→ and teichoic acid →1)-sn-Gro-(3-P-3)-β-[β-D-GlcpNAc-(1→2]-D-Galp-(1→. Two glycopolymers were identified in A. auranticolor VKM Ac-648T and A. cyaneus VKM Ac-1095T: minor polymer – unsubstituted 2,3-poly(glycerol phosphate), widely abundant in actinobacteria (Ac-648T), and mannan with trisaccharide repeating unit →2)-α-D-Manp-(1→2)-α-D-Manp-(1→6)-α-D-Manp-(1→ (Ac-1095T). In addition, both microorganisms contain a teichuronic acid of unique structure containing a pentasaccharide repeating unit with two residues of glucopyranose and three residues of diaminouronic acids in D-manno- and/or D-gluco-configuration. Each of the strains demonstrates peculiarities in the structure of teichuronic acid with respect to the ratio of diaminouronic acids and availability and location of O-methyl groups in glucopyranose residues. All investigated strains contain a unique set of glycopolymers in their cell walls with structures not described earlier for prokaryotes.
KEY WORDS: Actinoplanes, cell wall, teichoic acids, glycosyl-1-phosphate polymers, teichuronic acids, mannan, NMR spectroscopy

DOI: 10.1134/S0006297916090091