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REVIEW: Immuno-PCR: Achievements and Perspectives

D. Y. Ryazantsev*, D. V. Voronina, and S. K. Zavriev

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia; E-mail: d.yu.ryazantsev@gmail.com, szavriev@ibch.ru

* To whom correspondence should be addressed.

Received April 26, 2016; Revision received July 11, 2016
The immuno-PCR (iPCR) method combines advantages of enzyme-linked immunosorbent assay and polymerase chain reaction, which is used in iPCR as a method of “visualization” of antigen–antibody interaction. The use of iPCR provides classical PCR sensitivity to objects traditionally detected by ELISA. This method could be very sensitive and allow for detection of quantities of femtograms/ml order. However, iPCR is still not widely used. The aim of this review is to highlight the special features of the iPCR method and to show the main aspects of its development and application in recent years.
KEY WORDS: immuno-PCR, quantitative PCR, antibody, antigen, DNA–antibody conjugate

DOI: 10.1134/S0006297916130113