2State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Moscow Region, Russia; E-mail: firstname.lastname@example.org
* To whom correspondence should be addressed.
Received March 30, 2016; Revision received November 1, 2016
The Gram-negative bacterium Lysobacter sp. XL1 produces outer membrane vesicles that are heterogeneous in size, density, and protein composition. One of the subpopulations is secretory vesicles for lytic protease L5 of Lysobacter sp. XL1 (Kudryakova et al. (2015) FEMS Microbiol. Lett., 362, fnv137). Protein L5 was assumed to influence biogenesis of these secretory vesicles that contain it. Using a Pseudomonas fluorescens Q2-87/B expression system, it was shown that the recombinant L5 protein may act as a factor of vesicle biogenesis. This points to a possible involvement of L5 protein in Lysobacter sp. XL1 vesicle biogenesis. Furthermore, it was established that the main phospholipid of Lysobacter sp. XL1 vesicles is cardiolipin, and vesicles are formed predominantly of outer membrane regions enriched with this phospholipid. This indicates that cardiolipin participates in biogenesis of all vesicle subpopulations in Lysobacter sp. XL1.
KEY WORDS: factors of outer membrane vesicle biogenesis, bacteriolytic protease L5, cardiolipin, Lysobacter sp. XL1, lytic effect