Minicircle Kinetoplast Genome of Insect Trypanosomatid Leptomonas pyrrhocoris

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E. S. Gerasimov^1*, A. A. Gasparyan¹, I. A. Litus¹, M. D. Logacheva², and A. A. Kolesnikov^1*

¹Lomonosov Moscow State University, Faculty of Biology, 119991 Moscow, Russia; E-mail: aak330@ynadex.ru, jalgard@gmail.com

²Lomonosov Moscow State University, Belozersky Institute of Physico-Chemical Biology, 119991 Moscow, Russia

^* To whom correspondence should be addressed.

Received November 1, 2016; Revision received November 28, 2016

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We present here the structure of a minicircle population based on transcriptome sequencing of Leptomonas pyrrhocoris. We show that minicircle DNA molecules are dimeric. As in dixenous species, the entire molecule of minicircle DNA is transcribed. This is the first minicircle transcriptome of monoxenous trypanosomatid species determined using NGS technology.

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KEY WORDS: trypanosomatids, kinetoplast, minicircle DNA, transcriptome, assembly

DOI: 10.1134/S0006297917050054

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